Coding

Part:BBa_K3579000:Design

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 794


Design Notes

BsaI and BbsI restriction sites have been removed



Source

Cloning vector pRAB11 GenBank: JN635500.1

References